Scientific Journals


Selected Scientific Articles


Bueno, R.† Marciello, M.,† Moreno, M., Sanchez-Sanchez, C., Martinez, J.I., Martinez, L., Prats-Alfonso, E., Guimera-Brunet, A., Garrido, J.A., Villa, R., Mompean, F., García-Hernandez, M. Huttel, Y., Morales, M. P., Briones, C., Lopez, M.F., Ellis, G.J., Vazquez, L., Martín-Gago, J.A. Versatile Graphene-Based Platform for Robust Nanobiohybrid Interfaces. ACS Omega, 2019, 4 (2), 3287–3297. †: Contributed equally.

Carceller, J. M., Martínez Galán, J. P., Monti, R., Bassan, J. C., Filice, M., Iborra, S., Yu, J., Corma, A. Selective synthesis of citrus flavonoids prunin and naringenin using heterogeneized biocatalyst on graphene oxide. Green Chem. 2019,21, 839-849.


Ramirez-Carracedo, R.; Tesoro, L.; Hernandez, I.; Diez-Mata, J.; Filice, M.; Toro, R.; Rodriguez-Piñero, M.; Zamorano, J.L.; Saura, M.; Zaragoza, C. Non-invasive detection of extracellular matrix metalloproteinase inducer EMMPRIN, a new therapeutic target against atherosclerosis, inhibited by endothelial nitric oxide. Int. J. Mol. Sci. 2018, 19, 3248.

Sánchez, A.; Ovejero, K.; Ruiz-Cabello, J.; Martinez-Ruiz, P.; Pingarron, J.M.; Villalonga R.; Filice, M.* Hybrid Decorated Core@Shell Janus Nanoparticles As Flexible Platform For Targeted Multimodal Molecular Bioimaging Of Cancer. ACS Appl. Mater. Interfaces, 2018, 10 (37), 31032–31043.

Viñambres, M.; Filice, M.;*  Marciello, M.* Modulation of the catalytic properties of lipase B from candida antarctica by immobilization on tailor-made magnetic iron oxide nanoparticles: The key role of nanocarrier surface engineering. Polymers 2018, 10(6).

Facchini, F. D. A.; Pereira, M. G.; Vici, A. C.; Filice, M.; Pessela, B. C.; Guisan, J. M.; Fernandez-Lorente, G; Polizeli, M. L. T. M. Immobilization effects on the catalytic properties of two fusarium verticillioides lipases: Stability, hydrolysis, transesterification and enantioselectivity improvement. Catalysts 2018, 8(2).

Erami, R. S.; Ovejero, K.; Meghdadi, S.; Filice, M.;* Amirnasr, M.; Rodríguez-Diéguez, A.; De La Orden, M.U.; Gómez-Ruiz, S. Applications of nanomaterials based on magnetite and mesoporous silica on the selective detection of zinc ion in live cell imaging. Nanomaterials, 20188(6).

Herranz, S.; Marciello, M.; Marco, M.; Garcia-Fierro, J. L.; Guisan, J. M.; Moreno-Bondi, M. C. Multiplex environmental pollutant analysis using an array biosensor coated with chimeric hapten-dextran-lipase constructs. Sensors and Actuators, B: Chemical, 2018257, 256-262.


Callaghan, C.; Redmond, M.; Alnoch, R. C.; Mateo, C.; Filice, M.;* Palomo, J. M. Biocatalytic process optimization for the production of high-added-value 6-O-hydroxy and 3-O-hydroxy glycosyl building blocksChemCatChem, 20179(13), 2536-2543.

Filice, M.; Aragon, C. C.; Mateo, C.; Palomo, J. M. Enzymatic transformations in food chemistry. Current Organic Chemistry, 201721(2), 139-148.

Filice, M.; Molina, M.; Benaiges, M. D.; Abian, O.; Valero, F.; Palomo, J. M. Solid-surface activated recombinant: Rhizopous oryzae lipase expressed in pichia pastoris and chemically modified variants as efficient catalysts in the synthesis of hydroxy monodeprotected glycals. Catalysis Science and Technology, 20177(8), 1766-1775.

Marciello, M.;* Rossi, S.; Caramella, C.; Remuñán-López, C. Freeze-dried cylinders carrying chitosan nanoparticles for vaginal peptide delivery. Carbohydrate Polymers, 2017170, 43-51.

Rojas, J. M.; Gavilán, H.; del Dedo, V.; Lorente-Sorolla, E.; Sanz-Ortega, L.; da Silva, G. B.; Costo, R.; Perez-Yagüe, S.; Talelli, M.; Marciello, M.Morales, M.P.; Barber, D.F.; Gutiérrez, L. Time-course assessment of the aggregation and metabolization of magnetic nanoparticles. Acta Biomaterialia, 201758, 181-195.

Da Silva, G. B.; Marciello, M.; Morales, M. D. P.; Serna, C. J.; Vargas, M. D.; Ronconi, C. M.; Costo, R. Studies of the colloidal properties of superparamagnetic iron oxide nanoparticles functionalized with platinum complexes in aqueous and PBS buffer media. Journal of the Brazilian Chemical Society, 201728(5), 731-739.

Pacakova, B.; Kubickova, S.; Salas, G.; Mantlikova, A. R.; Marciello, M.; Morales, M. P.; Niznansky, D.; Vejpravova, J. The internal structure of magnetic nanoparticles determines the magnetic response. Nanoscale, 20179(16), 5129-5140.

Alvarez-Sola, G.; Uriarte, I.; Latasa, M. U.; Fernandez-Barrena, M. G.; Urtasun, R.; Elizalde, M.; Barcena-Varela, M.; Jiménez, M.; Chang, H.C.; . . . Avila, M. A. Fibroblast growth factor 15/19 (FGF15/19) protects from diet-induced hepatic steatosis: Development of an FGF19-based chimeric molecule to promote fatty liver regeneration. Gut, 201766(10), 1818-1828.

Argemí, J.; Kress, T. R.; Chang, H. C. Y.; Ferrero, R.; Bértolo, C.; Moreno, H.; . . . Aragón, T. X-box binding protein 1 regulates unfolded protein, acute-phase, and DNA damage responses during Regeneration of mouse liver. Gastroenterology, 2017152(5), 1203-1216.


Marciello, M.; Pellico, J.; Fernandez-Barahona, I.; Herranz, F.; Ruiz-Cabello, J.; Filice, M.* Recent advances in the preparation and application of multifunctional iron oxide and liposome-based nanosystems for multimodal diagnosis and therapy. Interface Focus, 2016, 6(6).

Cuenca, T.; Filice, M.;* Palomo, J. M. Palladium nanoparticles enzyme aggregate (PANEA) as efficient catalyst for Suzuki–Miyaura reaction in aqueous media. Enzyme and Microbial Technology, 201695, 242-247.

Bassan, J. C.; Bezerra, T. M. S.; Peixoto, G.; Da Cruz, C. Z. P.; Galán, J. P. M.; Vaz, A. B. S.; Garrido, S.S.; Filice, M.; Monti, R. Addendum: Immobilization of trypsin in lignocellulosic waste material to produce peptides with bioactive potential from whey protein. [materials, 9, 5, (2016), (357)]. Materials, 20169(8).

Filice, M.* The lab-on-a-protein concept protein as powerful nanometric laboratory for chemical sciences. Current Organic Chemistry, 201620(11), 1149-1149.

Palomo, J. M.; Filice, M.* Biosynthesis of metal nanoparticles: Novel efficient heterogeneous nanocatalysts. Nanomaterials, 20166(5).

Aragon, C. C.; Palomo, J. M.; Filice, M.; Mateo, C. Immobilization of aldolase for C-C bond formation. Current Organic Chemistry, 201620(11). 1243-1251.

Bassan, J. C.; Bezerra, T. M. S.; Peixoto, G.; da Cruz, C. Z. P.; Galán, J. P. M.; Vaz, A. B. S.; Garrido, S.S.; Filice, M.; Monti, R. Immobilization of trypsin in lignocellulosic waste material to produce peptides with bioactive potential from whey protein. Materials, 2016, 9(5).

Simeonidis, K.; Morales, M. P.; Marciello, M.; Angelakeris, M.; De La Presa, P.; Lazaro-Carrillo, A.; Tabero, A.; Villanueva, A.; Chubykalo-Fesenko, O.; Serantes, D. In-situ particles reorientation during magnetic hyperthermia application: Shape matters twiceScientific Reports, 20166.

Zahraei, M.; Marciello, M.; Lazaro-Carrillo, A.; Villanueva, A.; Herranz, F.; Talelli, M.; Costo, R.; Monshi, A.; Shahbazi-Gahrouei, D.; Amirnasr, M.; Behdadfar, B.; Morales, M. P. Versatile theranostics agents designed by coating ferrite nanoparticles with biocompatible polymersNanotechnology, 201627(25).

Talelli, M.; Aires, A.; Marciello, M. Protein-modified magnetic nanoparticles for biomedical applications. Current Organic Chemistry, 201620(11), 1252-1261.

Coral, D. F.; Mendoza Zélis, P.; Marciello, M.; Morales, M. D. P.; Craievich, A.; Sánchez, F. H.; Fernández Van Raap, M. B. Effect of nanoclustering and dipolar interactions in heat generation for magnetic hyperthermiaLangmuir, 201632(5), 1201-1213.


Moreno-Perez, S.; Ghattas, N.; Filice, M.; Guisan, J. M.; Fernandez-Lorente, G. Dramatic hyperactivation of lipase of thermomyces lanuginosa by a cationic surfactant: Fixation of the hyperactivated form by adsorption on sulfopropyl-sepharose. Journal of Molecular Catalysis B: Enzymatic, 2015122, 199-203.

Filice, M.;* Romero, O.; Aires, A.; Guisan, J. M.; Rumbero, A.; Palomo, J. M. Preparation of an immobilized lipase-palladium artificial metalloenzyme as catalyst in the heck reaction: Role of the solid phase. Advanced Synthesis and Catalysis, 2015357(12), 2687-2696.

Filice, M.;* Romero, O.; Gutiérrez-Fernández, J.; De Las Rivas, B.; Hermoso, J. A.; Palomo, J. M. Synthesis of a heterogeneous artificial metallolipase with chimeric catalytic activity. Chemical Communications, 201551(45), 9324-9327.

Palomo, J. M.; Filice, M. New emerging bio-catalysts design in biotransformations. Biotechnology Advances, 201533(5), 605-613.

Palma, S. I. C. J.; Carvalho, A.; Silva, J.; Martins, P.; Marciello, M.; Fernandes, A. R.; Morales, M.D.P; Roque, A. C. A. Covalent coupling of gum arabic onto superparamagnetic iron oxide nanoparticles for MRI cell labeling: Physicochemical and in vitro characterization. Contrast Media and Molecular Imaging, 201510(4), 320-328.

Quintana, P. G.; Canet, A.; Marciello, M.; Valero, F.; Palomo, J. M.; Baldessari, A. Enzyme-catalyzed preparation of chenodeoxycholic esters by an immobilized heterologous Rhizopus oryzae lipase. Journal of Molecular Catalysis B: Enzymatic, 2015118, 36-42.

Branca, M.; Marciello, M.; Ciuculescu-Pradines, D.; Respaud, M.; Morales, M. D. P.; Serra, R.; Casanove, M.-J.; Amiens, C. Towards MRI T2 contrast agents of increased efficiency. Journal of Magnetism and Magnetic Materials, 2015377, 348-353.

Palma, S. I. C. J.; Marciello, M.; Carvalho, A.; Veintemillas-Verdaguer, S.; Morales, M. D. P.; Roque, A. C. A. Effects of phase transfer ligands on monodisperse iron oxide magnetic nanoparticles. Journal of Colloid and Interface Science, 2015437, 147-155.

Orbe, J.; Rodríguez, J. A.; Sánchez-Arias, J. A.; Salicio, A.; Belzunce, M.; Ugarte, A.; Chang, H.C.Y.; . . . Páramo, J. A. Discovery and safety profiling of a potent preclinical candidate, (4-[4-[[(3 R)-3-(hydroxycarbamoyl)-8-azaspiro[4.5]decan-3-yl]sulfonyl]phenoxy]- N -methylbenzamide) (CM-352), for the prevention and treatment of hemorrhage. Journal of Medicinal Chemistry, 201558(7), 2941-2957.


Filice, M.;* Palomo, J. M. Cascade reactions catalyzed by bionanostructures. ACS Catalysis, 20144(5), 1588-1598.

Ghattas, N.; Filice, M.; Abidi, F.; Guisan, J. M.; Ben Salah, A. Purification and improvement of the functional properties of Rhizopus oryzae lipase using immobilization techniques. Journal of Molecular Catalysis B: Enzymatic, 2014110, 111-116.

Filice, M.; Romero, O.; Abian, O.; De Las Rivas, B.; Palomo, J. M. Low ionic liquid concentration in water: A green and simple approach to improve activity and selectivity of lipases. RSC Advances, 20144(90), 49115-49122.

Marciello, M.; Filice, M.; Olea, D.; Velez, M.; Guisan, J. M.; Mateo, C. Useful oriented immobilization of antibodies on chimeric magnetic particles: Direct correlation of biomacromolecule orientation with biological activity by AFM studies. Langmuir, 201430(49), 15022-12030.

Bavaro, T.; Filice, M.;* Temporini, C.; Tengattini, S.; Serra, I.; Morelli, C. F.; Massolini, G; Terreni, M. Chemoenzymatic synthesis of neoglycoproteins driven by the assessment of protein surface reactivity. RSC Advances, 20144(99), 56455-56465.

Serantes, D.; Simeonidis, K.; Angelakeris, M.; Chubykalo-Fesenko, O.; Marciello, M.; Morales, M.D.P.; Baldomir, D.; Martinez-Boubeta, C. Multiplying magnetic hyperthermia response by nanoparticle assembling. Journal of Physical Chemistry C, 2014118(11), 5927-5934.

Veintemillas-Verdaguer, S.; Marciello, M.; Morales, M. D. P.; Serna, C. J.; Andrés-Vergés, M. Magnetic nanocrystals for biomedical applications. Progress in Crystal Growth and Characterization of Materials, 201460(3-4), 80-86.

Kossatz, S.; Ludwig, R.; Dähring, H.; Ettelt, V.; Rimkus, G.; Marciello, M.; Salas, G.Patel, V.; Teran, F.J.; Hilger, I. High therapeutic efficiency of magnetic hyperthermia in xenograft models achieved with moderate temperature dosages in the tumor area. Pharmaceutical Research, 201431(12), 3274-3288.


Brabcova, J.; Filice, M.; Gutarra, M.; Palomo, J. M. Synthesis of enantiopure drugs and drug intermediates by immobilized lipase-catalysis. Current Bioactive Compounds, 20139(2), 113-136.

Filice, M.;* Marciello, M.; Morales, M. D. P.; Palomo, J. M. Synthesis of heterogeneous enzyme-metal nanoparticle biohybrids in aqueous media and their applications in C-C bond formation and tandem catalysisChemical Communications, 201349(61), 6876-6878.

Filice, M.;* Marciello, M. Enzymatic synthesis of oligosaccharides: A powerful tool for a sweet challenge. Current Organic Chemistry, 201317(7), 701-718.

Marciello, M.; Bolivar, J. M.; Filice, M.; Mateo, C.; Guisan, J. M. Preparation of lipase-coated, stabilized, hydrophobic magnetic particles for reversible conjugation of biomacromolecules. Biomacromolecules, 201314(3), 602-607.

Moreno-Perez, S.; Filice, M.; Guisan, J. M.; Fernandez-Lorente, G. Synthesis of ascorbyl oleate by transesterification of olive oil with ascorbic acid in polar organic media catalyzed by immobilized lipases. Chemistry and Physics of Lipids, 2013174, 48-54.

Marciello, M.; Connord, V.; Veintemillas-Verdaguer, S.; Vergés, M. A.; Carrey, J.; Respaud, M.; Serna, C.J.; Morales, M. P. Large scale production of biocompatible magnetite nanocrystals with high saturation magnetization values through green aqueous synthesis. Journal of Materials Chemistry B, 20131(43), 5995-6004.

Herranz, S.; Marciello, M.; Olea, D.; Hernández, M.; Domingo, C.; Vélez, M.; Gheber, L.A.; Guisán, J.M.; Moreno-Bondi, M. C. Dextran-lipase conjugates as tools for low molecular weight ligand immobilization in microarray developmentAnalytical Chemistry, 201385(15), 7060-7068.

Bernal, C.; Marciello, M.; Mesa, M.; Sierra, L.; Fernandez-Lorente, G.; Mateo, C.; Guisan, J. M. Immobilisation and stabilisation of β-galactosidase from kluyveromyces lactis using a glyoxyl support. International Dairy Journal, 201328(2), 76-82.

Uriarte, I.; Fernandez-Barrena, M. G.; Monte, M. J.; Latasa, M. U.; Chang, H. C. Y.; Carotti, S.; . . . Avila, M. A. Identification of fibroblast growth factor 15 as a novel mediator of liver regeneration and its application in the prevention of post-resection liver failure in mice. Gut, 201362(6), 899-910.

Reboredo, M.; Chang, H. C. Y.; Barbero, R.; Rodríguez-Ortigosa, C. M.; Pérez-Vizcaíno, F.; Morán, A.; . . . Sangro, B. Zolmitriptan: A novel portal hypotensive agent which synergizes with propranolol in lowering portal pressure. PLoS ONE, 20138(1).


Romero, O.; Filice, M.; De Las Rivas, B.; Carrasco-Lopez, C.; Klett, J.; Morreale, A.; Hermoso, J.A.; Guisan, J.M.; Abian, O.; Palomo, J. M. Semisynthetic peptide-lipase conjugates for improved biotransformations. Chemical Communications, 201248(72), 9053-9055.

Filice, M.; Guisan, J. M.; Terreni, M.; Palomo, J. M. Regioselective monodeprotection of peracetylated carbohydrates. Nature Protocols, 20127(10), 1783-1796.

Marciello, M.; Filice, M.; Palomo, J. M. Different strategies to enhance the activity of lipase catalysts. Catalysis Science and Technology, 20122(8), 1531-1543.

Filice, M.;* Palomo, J. M. Monosaccharide derivatives as central scaffolds in the synthesis of glycosylated drugs. RSC Advances, 20122(5), 1729-1742.

Quintana, P. G.; Guillén, M.; Marciello, M.; Valero, F.; Palomo, J. M.; Baldessari, A. Immobilized heterologous Rhizopus oryzae lipase as an efficient catalyst in the acetylation of cortexolone. European Journal of Organic Chemistry, 2012(23), 4306-4312.

Rossi, S.; Marciello, M.; Ferrari, F.; Puccio, A.; Bonferoni, C.; Sandri, G.; Caramella, C. Development of sponge-like dressings for mucosal/transmucosal drug delivery into vaginal cavity. Pharmaceutical Development and Technology, 201217(2), 219-226.

Fernández-Barrena, M. G.; Monte, M. J.; Latasa, M. U.; Uriarte, I.; Vicente, E.; Chang, H. C. Y.; . . . Ávila, M. A. Lack of Abcc3 expression impairs bile-acid induced liver growth and delays hepatic regeneration after partial hepatectomy in mice. Journal of Hepatology, 201256(2), 367-373.


Bavaro, T.; Filice, M.; Bonomi, P.; Abu Alassal, Q.; Speranza, G.; Guisan, J. M.; Terreni, M. Regioselective deprotection of peracetylated disaccharides at the primary position catalyzed by immobilized acetyl xylan esterase from Bacillus pumilus. European Journal of Organic Chemistry, 201131, 6181-6185.

Pérez, D.; Martín, S.; Fernández-Lorente, G.; Filice, M.; Guisán, J. M.; Ventosa, A.; García, M.T.; Mellado, E. A novel halophilic lipase, LipBL, showing high efficiency in the production of eicosapentaenoic acid (EPA). PLoS ONE, 20116(8).

Filice, M.; Romero, O.; Guisan, J. M.; Palomo, J. M. Trans,trans-2,4-hexadiene incorporation on enzymes for site-specific immobilization and fluorescent labeling. Organic and Biomolecular Chemistry, 20119(15), 5535-5540.

Godoy, C. A.; Fernández-Lorente, G.; De Las Rivas, B.; Filice, M.; Guisan, J. M.; Palomo, J. M. Medium engineering on modified Geobacillus thermocatenulatus lipase to prepare highly active catalysts. Journal of Molecular Catalysis B: Enzymatic, 201170(3-4), 144-148.

Filice, M.; Marciello, M.; Betancor, L.; Carrascosa, A. V.; Guisan, J. M.; Fernandez-Lorente, G. Hydrolysis of fish oil by hyperactivated Rhizomucor miehei lipase immobilized by multipoint anion exchange. Biotechnology Progress, 201127(4), 961-968.

Fernandez-Lorente, G.; Filice, M.; Lopez-Vela, D.; Pizarro, C.; Wilson, L.; Betancor, L.; Avila, Y.; Guisan, J. M. Cross-linking of lipases adsorbed on hydrophobic supports: Highly selective hydrolysis of fish oil catalyzed by RML. JAOCS, Journal of the American Oil Chemists' Society, 201188(6), 801-807.

Volpato, G.; Filice, M.; de las Rivas, B.; Rodrigues, R. C.; Heck, J. X.; Fernandez-Lafuente, R.; Guisan, J.M.; Mateo, C.; Ayub, M. A. Z. Purification, immobilization, and characterization of a specific lipase from Staphylococcus warneri EX17 by enzyme fractionating via adsorption on different hydrophobic supports. Biotechnology Progress, 201127(3), 717-723.

Acosta, A.; Filice, M.; Fernandez-Lorente, G.; Palomo, J. M.; Guisan, J. M. Kinetically controlled synthesis of monoglyceryl esters from chiral and prochiral acids methyl esters catalyzed by immobilized Rhizomucor miehei lipase. Bioresource Technology, 2011102(2), 507-512.

Marciello, M.; Mateo, C.; Guisan, J. M. Full enzymatic hydrolysis of commercial sucrose laurate by immobilized-stabilized derivatives of lipase from Thermomyces lanuginosa. Colloids and Surfaces B: Biointerfaces, 201184(2), 556-560.

Rossi, S.; Marciello, M.; Bonferoni, M. C.; Ferrari, F.; Sandri, G.; Dacarro, C.; Grisoli, P.; Caramella, C. Thermally sensitive gels based on chitosan derivatives for the treatment of oral mucositis. European Journal of Pharmaceutics and Biopharmaceutics, 201074(2), 248-254.


Filice, M.; Guisan, J. M.; Palomo, J. M. Effect of ionic liquids as additives in the catalytic properties of different immobilized preparations of Rhizomucor miehei lipase in the hydrolysis of peracetylated lactal. Green Chemistry, 201012(8), 1365-1369.

Filice, M.; Guisan, J. M.; Palomo, J. M. Recent trends in regioselective protection and deprotection of monosaccharides. Current Organic Chemistry, 201014(6), 516-532.

Godoy, C. A.; de las Rivas, B.; Filice, M.; Fernández-Lorente, G.; Guisan, J. M.; Palomo, J.  Enhanced activity of an immobilized lipase promoted by site-directed chemical modification with polymers. Process Biochemistry, 201045(4), 534-541.

Volpato, G.; Filice, M.; Ayub, M. A. Z.; Guisan, J. M.; Palomo, J. M. Single-step purification of different lipases from Staphylococcus warneri. Journal of Chromatography A, 20101217(4), 473-478.